Services/Pricing (Download PDF)

NEW in 2023-2024: We are migrating higher depth amplicons (2X) to the NextSeq2000 to provide greater quality.


Note: All prices listed are in Canadian Dollars (CAD). International orders must add a fee of $25/shipment to cover Customs costs.

For a listing of currently available Amplicon Targets (and primer sets), see our Protocols page here.

 16S/18S/ITS Short Amplicons (per sample, per amplicon) - MiSeq (2x300 bp PE) - Academic/Govt.

<50 samples 50-200 samples >200 samples1
Library Preparation + Sequencing (max. 50k reads/sample2) $30 $25 $20
      or 2X depth (max. 100k reads/sample2) $45 $38 $30
DNA Extraction3 $15 $15 $15
Standard Bioinformatics Analysis Pipeline4 $500/project$750/project $1000/project

 16S/18S/ITS Short Amplicons (per sample, per amplicon) - MiSeq (2x300 bp PE) - Commercial

<50 samples 50-200 samples >200 samples1
Library Preparation + Sequencing (max. 50k reads/sample2) $40 $35 $30
      or 2X depth (max. 100k reads/sample2) $60 $53 $45
DNA Extraction3 $20 $20 $20
Standard Bioinformatics Analysis Pipeline4 $750/project$1000/project $1500/project

 16S/18S/ITS Full-Length Amplicons (per sample, per amplicon) - PacBio Sequel2 (long reads) - Academic/Govt.

<50 samples 50-200 samples >200 samples1
Library Preparation + Sequencing (max. 10k CCS=HiFi reads/sample2) $35 $30 $25
      or 2X depth (max. 20k reads/sample2) $53 $45 $38
DNA Extraction3 $15 $15 $15
Standard Bioinformatics Analysis Pipeline4 $500/project$750/project $1000/project

 16S/18S/ITS Full-Length Amplicons (per sample, per amplicon) - PacBio Sequel2 (long reads) - Commercial

<50 samples 50-200 samples >200 samples1
Library Preparation + Sequencing (max. 10k CCS=HiFi reads/sample2) $50 $45 $40
      or 2X depth (max. 20k reads/sample2) $75 $67 $60
DNA Extraction3 $20 $20 $20
Standard Bioinformatics Analysis Pipeline4 $750/project$1000/project $1500/project
Note: PacBio pricing is currently introductory and could change within 2022 as we perfect the system/depths of coverage.
 

 Client-Prepared Pool of Amplicons (per whole run) - MiSeq (2x300 bp or 2x150 bp PE)

Academic/Govt. Commercial
Per run Per run
MiSeq Standard Run (~20-25 M PE reads & 12-15 Gb) $3000 $4500
MiSeq Micro 2x150 bp Run (~4 M PE reads & 1.2 Gb) $950 $1400
MiSeq Nano 2x150 bp Run (~1 M PE reads & 0.3 Gb) $700 $1000
Note: For clients wishing to use part of a standard run with a prepared pool of amplicons, simply divide 380 by your sample # x the above price to determine your pool cost (this is for a 1X depth, simply double the amount for 2X).

 Client-Prepared Pool of Metagenomes (per whole run) - NextSeq2000 (2x150 bp PE)

Academic/Govt. Commercial
Per run Per run
NextSeq2000 P1 cell Run (~100 M PE reads = 200 M single reads & 30 Gb) $2400 $3000
NextSeq2000 P2 cell Run (~400 M PE reads = 800 M single reads & 120 Gb) $6500 $7000
NextSeq2000 P3 cell Run (~1.2 B PE reads = 2.4 B single reads & 360 Gb) $10000 $10500
Note: For clients wishing to use part of a run with a prepared pool of metagenomes, simply divide 96 by your sample # x the above price to determine your pool cost (this is for a 1X depth).
 

 Viral/Phage Genomes (~1000X typical 150 kb genome) - MiSeq (2x300 bp PE) - De novo Assembly

Academic/Govt. Commercial
Per genome Per genome
Library Prep. + Sequencing (~0.25 M PE reads = 0.5 M single reads & 150 Mb/genome) $100 $150
Custom Bioinformatics Analysis inquire inquire

 Prokaryotic Genomes (~100X typical 5 Mb genome) - MiSeq (2x300 bp PE) - Resequencing

Academic/Govt. Commercial
Per run of 24 Per genome Per run of 24 Per genome
Library Prep. + Sequencing (~0.9 M PE reads = 1.8 M single reads & 540 Mb/genome) $6000 $250 $8400 $350
Custom Bioinformatics Analysis inquire inquire

 Prokaryotic Genomes (~400X typical 5 Mb genome) - PacBio Sequel2 (long reads) - De novo Assembly

Academic/Govt. Commercial
Per run of 48 Per genome Per run of 48 Per genome
Library Prep. + Sequencing (~2 Gb/genome) $10800 $225 $16320 $340
Custom Bioinformatics Analysis inquire inquire
Note: PacBio pricing is currently introductory and could change within 2022 as we perfect the system/depths of coverage.

 (Micro)Eukaryote Genomes (100 Gb per cell) - PacBio Sequel2 (long reads) - De novo Assembly

Academic/Govt. Commercial
Per run of (X) Per genome Per run of (X) Per genome
Library Prep. + Sequencing (~50 Gb/genome) $3200 (2) $1600 $4800 (2) $2400
      or whole cell (~100 Gb/genome) $3000 (1) $3000 $4500 (1) $4500
Custom Bioinformatics Analysis inquire inquire
Note: PacBio pricing is currently introductory and could change within 2022 as we perfect the system/depths of coverage.
 

 Metagenomes - NextSeq2000 (2x150 bp PE P3 cell) - Profiling and Contig Assembly

Academic/Govt. Commercial
Per run of (X) Per sample Per run of (X) Per sample
Library Prep. + Sequencing (~6 M PE reads = 12 M single reads & 1.8 Gb/sample) $24960 (192) $130 $38400 (192) $200
      or 2X depth (~12 M PE reads = 24 M single reads & 3.6 Gb/sample) $19200 (96) $200 $28800 (96) $300
      or 4X depth (~24 M PE reads = 48 M single reads & 7.2 Gb/sample) $14400 (48) $300 $21600 (48) $450
      or 6X depth (~36 M PE reads = 72 M single reads & 11 Gb/sample) $13600 (32) $425 $20800 (32) $650
      or 8X depth (~48 M PE reads = 96 M single reads & 14 Gb/sample) $13200 (24) $550 $19800 (24) $825
      or 12X depth (~72 M PE reads = 144 M single reads & 22 Gb/sample) $12000 (16) $750 $18000 (16) $1125
      or 16X depth (~96 M PE reads = 192 M single reads & 29 Gb/sample) $11700 (12) $975 $17400 (12) $1450
      or 24X depth (~144 M PE reads = 288 M single reads & 43 Gb/sample) $11400 (8) $1425 $17200 (8) $2150
      or 32X depth (~192 M PE reads = 384 M single reads & 58 Gb/sample) $11400 (6) $1900 $17100 (6) $2850
DNA Extraction3 - $15 - $20
Standard Bioinformatics Analysis Pipeline5 $1000/project $1500/project

 Metagenomes - PacBio Sequel2 (long reads) - Shallow Profiling

Academic/Govt. Commercial
Per run of 48 Per sample Per run of 48 Per sample
Library Prep. + Sequencing (~2 Gb/sample) $10800 $225 $16320 $340
DNA Extraction3 - $15 - $20
Custom Bioinformatics Analysis inquire inquire
Note: PacBio pricing is currently introductory and could change within 2022 as we perfect the system/depths of coverage.

 Metagenomes - PacBio Sequel2 (long reads) - MAG Assembly

Academic/Govt. Commercial
Per run of (X) Per sample Per run of (X) Per sample
Library Prep. + Sequencing (~50 Gb/sample) $3200 (2) $1600 $4800 (2) $2400
      or whole cell (~100 Gb/sample) $3000 (1) $3000 $4500 (1) $4500
DNA Extraction3 - $15 - $20
Custom Bioinformatics Analysis inquire inquire
Note: PacBio pricing is currently introductory and could change within 2022 as we perfect the system/depths of coverage.
 

 (Meta)transcriptomes - NextSeq2000 (2x150 bp PE P3 cell) - Total Stranded RNA w/rRNA Depletion

Academic/Govt. Commercial
Per run of 48 Per sample Per run of 48 Per sample
Library Prep. + Sequencing (~24 M PE reads = 48 M single reads & 7.2 Gb/sample) $26400 $550 $38400 $800
RNA Extraction3 Not yet available Not yet available
Standard Bioinformatics Analysis Pipeline5 $1000/project $1500/project

 Iso-Seq - PacBio Sequel2 (long reads) - Full-Length Isoform Transcripts

Academic/Govt. Commercial
Per run of 8 Per sample Per run of 8 Per sample
Library Prep. + Sequencing (~12 Gb and ~500k reads/sample) $4000 $500 $6000 $750
RNA Extraction3 Not yet available Not yet available
Standard PacBio Iso-Seq Pipeline5 $1000/project $1500/project
Note: PacBio pricing is currently introductory and could change within 2022 as we perfect the system/depths of coverage.
 


Notes/Details

1. One control well is required per 96-well PCR library plate, hence a maximum of 4x95=380 samples can be done together on one MiSeq run (190 for a 2X run) or 2x95=190 samples on a Sequel2 run (95 for a 2X run). Any batches >380/190 will need to be spread across multiple MiSeq/Sequel2 runs.

2. These are generally maximum raw read outputs you will receive, but there is inherent variability in runs, meaning averages can be closer to 30k/60k (MiSeq 1X/2X) or 12k/24k (Sequel 2 1X/2X).

3. Extra costs may be incurred if samples, due to their nature, require more specialized (length, cost, etc.) extraction procedures.

4. & 5. Details of our amplicon and metagenomics pipelines are available at https://github.com/mlangill/microbiome_helper, but a summary of the major deliverables clients will receive (analyses will require a "mapping file" from the clients containing any relevant metadata for the study) are available on our Protocols page.

Custom Bioinformatics: Additional bioinformatic analyses can be requested at an hourly rate or through research collaboration. Please contact us for more details.

Customs primers/amplicons: Beyond the 16S/18S/ITS amplicons offered "in-stock", essentially any PCR amplicon (max. size ~550 bp, best ~450-500 bp for MiSeq; no size limit for Sequel 2) can be adapted to our systems. Please consults us for primer design/protocol details. Pricing is as follows (one-time extra oligo ordering charge): $750/1000/1500 for oligos for 1-95/96-190/191-380 samples for MiSeq; $250/500 for oligos for 1-95/96-190 samples for Sequel 2.

Processing time: Time to completion may be variable depending the amount of other partial projects in the queue - this generally no longer applies to amplicons as we are at capacity, but occurs more when submitting small numbers of samples for metagenomics runs (<<192 for NextSeq2000) or de novo genomes (<<48 for Sequel2).

Payment: Payment is required within 30 days of receiving your invoice, only after completion of work (ie: delivery of data). All payments are final and no refunds are accepted.