COVID-19 update (2022): The IMR remains OPEN, however global consumable shortages are affecting processing times (see queue page).

NEW: We have upgraded to a PacBio Sequel 2 and new pricing/depths are now posted for full-length amplicons, WGS and metagenomes!


Note: All prices listed are in Canadian Dollars (CAD). International orders must add a $25/shipment fee to cover Customs costs.

For a listing of currently available Amplicon Targets (and primer sets), see our Protocols page here.

 16S/18S/ITS Short Amplicons (per sample, per amplicon) - MiSeq (300+300 bp PE) - Academic/Govt.

<50 samples 50-200 samples >200 samples1
Library Preparation + Sequencing (max. 50k reads/sample2) $30 $25 $20
      or 2X depth (max. 100k reads/sample2) $45 $38 $30
DNA Extraction3 $15 $15 $15
Standard Bioinformatics Analysis Pipeline4 $500/project$750/project $1000/project

 16S/18S/ITS Short Amplicons (per sample, per amplicon) - MiSeq (300+300 bp PE) - Commercial

<50 samples 50-200 samples >200 samples1
Library Preparation + Sequencing (max. 50k reads/sample2) $40 $35 $30
      or 2X depth (max. 100k reads/sample2) $60 $53 $45
DNA Extraction3 $20 $20 $20
Standard Bioinformatics Analysis Pipeline4 $750/project$1000/project $1500/project

 16S/18S/ITS Full-Length Amplicons (per sample, per amplicon) - PacBio Sequel 2 (long reads) - Academic/Govt.

<50 samples 50-200 samples >200 samples1
Library Preparation + Sequencing (max. 10k CCS=HiFi reads/sample2) $35 $30 $25
      or 2X depth (max. 20k reads/sample2) $53 $45 $38
DNA Extraction3 $15 $15 $15
Standard Bioinformatics Analysis Pipeline4 $500/project$750/project $1000/project

 16S/18S/ITS Full-Length Amplicons (per sample, per amplicon) - PacBio Sequel 2 (long reads) - Commercial

<50 samples 50-200 samples >200 samples1
Library Preparation + Sequencing (max. 10k CCS=HiFi reads/sample2) $50 $45 $40
      or 2X depth (max. 20k reads/sample2) $75 $67 $60
DNA Extraction3 $20 $20 $20
Standard Bioinformatics Analysis Pipeline4 $750/project$1000/project $1500/project
Note: PacBio pricing is currently introductory and could change within 2022 as we perfect the system/depths of coverage.
 

 Client-Prepared Pool of Amplicons (per whole run) - MiSeq (300+300 bp PE)

Academic/Govt. Commercial
Per run Per run
MiSeq Sequencing Run (~20-25 M PE reads & 12-15 Gb) $3200 $4800
Note: For clients wishing to use part of a run with a prepared pool of amplicons, simply divide 380 by your sample # x the above price to determine your pool cost (this is for a 1X depth, simply double the amount for 2X).
 

 Client-Prepared Pool of Metagenomes (per whole run) - NextSeq (150+150 bp PE)

Academic/Govt. Commercial
Per run Per run
NextSeq Sequencing Run (~400 M PE reads = 800 M single reads & 120 Gb)$8000 $12000
Note: For clients wishing to use part of a run with a prepared pool of metagenomes, simply divide 96 by your sample # x the above price to determine your pool cost (this is for a 1X depth).
 

 Viral/Phage Genomes (~1000X typical 150 kb genome) - MiSeq (300+300 bp PE) - De novo Assembly

Academic/Govt. Commercial
Per genome Per genome
Library Prep. + Sequencing (~0.25 M PE reads = 0.5 M single reads & 150 Mb/genome)$100 $150
Custom Bioinformatics Analysis inquire inquire

 Prokaryotic Genomes (~100X typical 5 Mb genome) - MiSeq (300+300 bp PE) - Resequencing

Academic/Govt. Commercial
Per run of 24 Per genome Per run of 24 Per genome
Library Prep. + Sequencing (~0.9 M PE reads = 1.8 M single reads & 540 Mb/genome)$6000 $250 $8400 $350
Custom Bioinformatics Analysis inquire inquire

 Prokaryotic Genomes (~400X typical 5 Mb genome) - PacBio Sequel 2 (long reads) - De novo Assembly

Academic/Govt. Commercial
Per run of 48 Per genome Per run of 48 Per genome
Library Prep. + Sequencing (~2 Gb/genome) $10800 $225 $16320 $340
Custom Bioinformatics Analysis inquire inquire
Note: PacBio pricing is currently introductory and could change within 2022 as we perfect the system/depths of coverage.

 (Micro)Eukaryote Genomes (100 Gb per cell) - PacBio Sequel 2 (long reads) - De novo Assembly

Academic/Govt. Commercial
Per run of (X) Per genome Per run of (X) Per genome
Library Prep. + Sequencing (~50 Gb/genome) $3200 (2) $1600 $4800 (2) $2400
      or whole cell (~100 Gb/genome) $3000 (1) $3000 $4500 (1) $4500
Custom Bioinformatics Analysis inquire inquire
Note: PacBio pricing is currently introductory and could change within 2022 as we perfect the system/depths of coverage.
 

 Metagenomes - NextSeq (150+150 bp PE) - Regular Depths

Academic/Govt. Commercial
Per run of (X) Per sample Per run of (X) Per sample
Library Prep. + Sequencing (~4 M PE reads = 8 M single reads & 1.2 Gb/sample) $14400 (96) $150 $21120 (96) $220
      or 2X depth (~8 M PE reads = 16 M single reads & 2.4 Gb/sample) $12000 (48) $250 $15360 (48) $320
      or 3X depth (~12 M PE reads = 24 M single reads & 3.6 Gb/sample) $11200 (32) $350 $13440 (32) $420
      or 4X depth (~16 M PE reads = 32 M single reads & 4.8 Gb/sample) $10800 (24) $450 $12480 (24) $520
DNA Extraction3 - $15 - $20
Standard Bioinformatics Analysis Pipeline5 $1000/project $1500/project

 Metagenomes - NextSeq (150+150 bp PE) - High Depths

Academic/Govt. Commercial
Per run of (X) Per run of (X)
Library Prep. + Sequencing (Whole run = ~400 M PE reads = 800 M single reads & 120 Gb) - -
      <6 samples (80+ M PE reads = 160+ M single reads) $7000 (<6) $10500 (<6)
      6-8 samples (~50-67 M PE reads = 100-133 M single reads) $8000 (6-8) $12000 (6-8)
      9-10 samples (~40-44 M PE reads = 80-88 M single reads) $9000 (9-10) $13500 (9-10)
      11-12 samples (~33-36 M PE reads = 67-73 M single reads) $10000 (11-12) $15000 (11-12)
DNA Extraction3 $15/sample $20/sample
Standard Bioinformatics Analysis Pipeline5 $1000/project $1500/project

 Metagenomes - PacBio Sequel (long reads) - MAG Assembly

Academic/Govt. Commercial
Per run of (X) Per sample Per run of (X) Per sample
Library Prep. + Sequencing (~50 Gb/half-cell) $3200 (2) $1600 $4800 (2) $2400
      or whole cell (~100 Gb) $3000 (1) $3000 $4500 (1) $4500
DNA Extraction3 - $15 - $20
Custom Bioinformatics Analysis inquire inquire
Note: PacBio pricing is currently introductory and could change within 2022 as we perfect the system/depths of coverage.
 

 (Meta)transcriptomes - NextSeq (150+150 bp PE) - Total Stranded RNA w/rRNA Depletion

Academic/Govt. Commercial
Per run of 24 Per sample Per run of 24 Per sample
Library Prep. + Sequencing (~16 M PE reads = 32 M single reads & 4.8 Gb/sample) $13200 $550 $19200 $800
RNA Extraction3 Not yet available Not yet available
Standard Bioinformatics Analysis Pipeline5 $1000/project $1500/project

 Iso-Seq - PacBio Sequel 2 (long reads) - Full-Length Isoform Transcripts

Academic/Govt. Commercial
Per run of 8 Per sample Per run of 8 Per sample
Library Prep. + Sequencing (~12 Gb and ~500k reads/sample) $4000 $500 $6000 $750
RNA Extraction3 Not yet available Not yet available
Standard PacBio Iso-Seq Pipeline5 $1000/project $1500/project
Note: PacBio pricing is currently introductory and could change within 2022 as we perfect the system/depths of coverage.
 


Notes/Details

1. One control well is required per 96-well PCR library plate, hence a maximum of 4x95=380 samples can be done together on one MiSeq run (190 for a 2X run) or 2x95=190 samples on a Sequel 2 run (95 for a 2X run). Any batches >380/190 will need to be spread across mutliple MiSeq/Sequel 2 runs.

2. These are generally maximum raw read outputs you will received, but there is inherent variability in runs, meaning averages can be closer to 30k/60k (MiSeq 1X/2X) or 8k/16k (Sequel 2 1X/2X).

3. Extra costs may be incurred if samples, due to their nature, require more specialized (length, cost, etc.) extraction procedures.

4. & 5. Details of our amplicon and metagenomics pipelines are available at https://github.com/mlangill/microbiome_helper, but a summary of the major deliverables clients will receive (analyses will require a "mapping file" from the clients containing any relevant metadata for the study) are available on our Protocols page.

Custom Bioinformatics: Additional bioinformatic analyses can be requested at an hourly rate or through research collaboration. Please contact us for more details.

Customs primers/amplicons: Beyond the 16S/18S/ITS amplicons offered "in-stock", essentially any PCR amplicon (max. size ~550 bp, best ~450-500 bp for MiSeq; no size limit for Sequel 2) can be adapted to our systems. Please consults us for primer design/protocol details. Pricing is as follows (one-time extra oligo ordering charge): $750/1000/1500 for oligos for 1-95/96-190/191-380 samples for MiSeq; $250/500 for oligos for 1-95/96-190 samples for Sequel 2.

Processing time: Time to completion may be variable depending the amount of other partial projects in the queue - this generally so longer applies to amplicons as we are at capacity, but occurs more when submitting small numbers of samples for metagenomics runs (<<96 for NextSeq) or de novo genomes (<<48 for Sequel 2).

Payment: Payment is required within 30 days of receiving your invoice, only after completion of work (ie: delivery of data). All payments are final and no refunds are accepted.