Services/Pricing (Download PDF)
NEW pricing effective for all samples submitted after April 15th, 2024. Pricing tiers have been simplified; increases have been applied for the first time since 2019 due to higher costs and overhead charges.
NEW in 2024: We are migrating higher depth amplicons (2X) to the NextSeq2000 to provide greater quality. MGS pricing should decrease pending the release of new XLEAP chemistry mid-2024.
Note: All prices listed are in Canadian Dollars (CAD). GST/HST will be added to all Canadian orders. A fee of $25/shipment to cover Customs costs will be added to all international orders.
For a listing of currently available Amplicon Targets (and primer sets), see our Protocols page here.
16S/18S/ITS Short Amplicons (per sample, per amplicon) - MiSeq (2x300 bp PE) | ||
| Academic/Govt. | Commercial | |
|---|---|---|
| Per sample1 | Per sample1 | |
| Library Preparation + Sequencing (max. 50k reads/sample2) | $30 | $40 |
| or 2X depth (max. 100k reads/sample2) | $45 | $55 |
| DNA Extraction3 | $35 | $45 |
| Standard Bioinformatics Analysis Pipeline4 | $1000/project | $1500/project |
16S/18S/ITS Full-Length Amplicons (per sample, per amplicon) - PacBio Sequel2 (long reads) | ||
| Academic/Govt. | Commercial | |
|---|---|---|
| Per sample1 | Per sample1 | |
| Library Preparation + Sequencing (max. 10k CCS=HiFi reads/sample2) | $35 | $45 |
| or 2X depth (max. 20k reads/sample2) | $65 | $75 |
| DNA Extraction3 | $35 | $45 |
| Standard Bioinformatics Analysis Pipeline4 | $1000/project | $1500/project |
Client-Prepared Pool of Amplicons (per whole run) - MiSeq (150/250/300 PE) | ||
| Academic/Govt. | Commercial | |
|---|---|---|
| Per run | Per run | |
| MiSeq Nano 2x250 bp Run (~1 M PE reads & 0.3 Gb) | $2400 | $3000 |
| MiSeq Micro 2x150 bp Run (~4 M PE reads & 1.2 Gb) | $2600 | $3200 |
| MiSeq Standard 2x300 bp Run (~20-25 M PE reads & 12-15 Gb) | $6000 | $7000 |
Client-Prepared Pool of Metagenomes (per whole run) - NextSeq2000 (2x150 bp PE) | ||
| Academic/Govt. | Commercial | |
|---|---|---|
| Per run | Per run | |
| NextSeq2000 P1 cell Run (~100 M PE reads = 200 M single reads & 30 Gb) | $4700 | $5700 |
| NextSeq2000 P2 cell Run (~400 M PE reads = 800 M single reads & 120 Gb) | $11000 | $12000 |
| NextSeq2000 P3 cell Run (~1.2 B PE reads = 2.4 B single reads & 360 Gb) | $18000 | $19000 |
Viral/Phage Genomes (~1000X typical 150 kb genome) - MiSeq (2x300 bp PE) - De novo Assembly | ||||
| Academic/Govt. | Commercial | |||
|---|---|---|---|---|
| Per genome | Per genome | |||
| Library Prep. + Sequencing (~0.25 M PE reads = 0.5 M single reads & 150 Mb/genome) | $200 | $300 | ||
| DNA Extraction3 | $35 | $45 | ||
| Custom Bioinformatics Analysis | inquire | inquire | ||
Prokaryotic Genomes (~100X typical 5 Mb genome) - MiSeq (2x300 bp PE) - Resequencing | ||||
| Academic/Govt. | Commercial | |||
|---|---|---|---|---|
| Per run of 24 | Per genome | Per run of 24 | Per genome | |
| Library Prep. + Sequencing (~0.9 M PE reads = 1.8 M single reads & 540 Mb/genome) | $12000 | $500 | $14400 | $600 |
| DNA Extraction3 | - | $35 | - | $45 |
| Custom Bioinformatics Analysis | inquire | inquire | ||
Prokaryotic Genomes (~400X typical 5 Mb genome) - PacBio Sequel2 (long reads) - De novo Assembly | ||||
| Academic/Govt. | Commercial | |||
|---|---|---|---|---|
| Per run of 48 | Per genome | Per run of 48 | Per genome | |
| Library Prep. + Sequencing (~2 Gb/genome) | $14400 | $300 | $19200 | $400 |
| DNA Extraction3 | - | $35 | - | $45 |
| Custom Bioinformatics Analysis | inquire | inquire | ||
(Micro)Eukaryote Genomes (100 Gb per cell) - PacBio Sequel2 (long reads) - De novo Assembly | ||||
| Academic/Govt. | Commercial | |||
|---|---|---|---|---|
| Per run of (X) | Per genome | Per run of (X) | Per genome | |
| Library Prep. + Sequencing (~50 Gb/genome) | $6000 (2) | $3000 | $8000 (2) | $4000 |
| or whole cell (~100 Gb/genome) | $5500 (1) | $5500 | $6500 (1) | $6500 |
| DNA Extraction3 | - | $35 | - | $45 |
| Custom Bioinformatics Analysis | inquire | inquire | ||
Metagenomes - NextSeq2000 (2x150 bp PE P3 cell) - Profiling and Contig Assembly | ||||
| Academic/Govt. | Commercial | |||
|---|---|---|---|---|
| Per run of (X) | Per sample | Per run of (X) | Per sample | |
| Library Prep. + Sequencing (~6 M PE reads = 12 M single reads & 1.8 Gb/sample) | $42240 (192) | $220 | $57600 (192) | $300 |
| or 2X depth (~12 M PE reads = 24 M single reads & 3.6 Gb/sample) | $31200 (96) | $325 | $38400 (96) | $400 |
| or 4X depth (~24 M PE reads = 48 M single reads & 7.2 Gb/sample) | $25200 (48) | $525 | $33600 (48) | $700 |
| or 6X depth (~36 M PE reads = 72 M single reads & 11 Gb/sample) | $24000 (32) | $750 | $32000 (32) | $1000 |
| or 8X depth (~48 M PE reads = 96 M single reads & 14 Gb/sample) | $22800 (24) | $950 | $28800 (24) | $1200 |
| or 12X depth (~72 M PE reads = 144 M single reads & 22 Gb/sample) | $22400 (16) | $1400 | $28800 (16) | $1800 |
| or 16X depth (~96 M PE reads = 192 M single reads & 29 Gb/sample) | $22200 (12) | $1850 | $28800 (12) | $2400 |
| or 24X depth (~144 M PE reads = 288 M single reads & 43 Gb/sample) | $21600 (8) | $2700 | $28000 (8) | $3500 |
| or 32X depth (~192 M PE reads = 384 M single reads & 58 Gb/sample) | $21600 (6) | $3600 | $27600 (6) | $4600 |
| DNA Extraction3 | - | $35 | - | $45 |
| Standard Bioinformatics Analysis Pipeline5 | $1000/project | $1500/project | ||
Metagenomes - PacBio Sequel2 (long reads) - Shallow Profiling | ||||
| Academic/Govt. | Commercial | |||
|---|---|---|---|---|
| Per run of 48 | Per sample | Per run of 48 | Per sample | |
| Library Prep. + Sequencing (~2 Gb/sample) | $14400 | $300 | $19200 | $400 |
| DNA Extraction3 | - | $35 | - | $45 |
| Custom Bioinformatics Analysis | inquire | inquire | ||
Metagenomes - PacBio Sequel2 (long reads) - MAG Assembly | ||||
| Academic/Govt. | Commercial | |||
|---|---|---|---|---|
| Per run of (X) | Per sample | Per run of (X) | Per sample | |
| Library Prep. + Sequencing (~50 Gb/sample) | $6000 (2) | $3000 | $8000 (2) | $4000 |
| or whole cell (~100 Gb/sample) | $5500 (1) | $5500 | $6500 (1) | $6500 |
| DNA Extraction3 | - | $35 | - | $45 |
| Custom Bioinformatics Analysis | inquire | inquire | ||
(Meta)transcriptomes - NextSeq2000 (2x150 bp PE P3 cell) - Total Stranded RNA w/rRNA Depletion | ||||
| Academic/Govt. | Commercial | |||
|---|---|---|---|---|
| Per run of 48 | Per sample | Per run of 48 | Per sample | |
| Library Prep. + Sequencing (~24 M PE reads = 48 M single reads & 7.2 Gb/sample) | $31200 | $650 | $38400 | $800 |
| RNA Extraction3 | Not yet available | Not yet available | ||
| Standard Bioinformatics Analysis Pipeline5 | $1000/project | $1500/project | ||
Iso-Seq - PacBio Sequel2 (long reads) - Full-Length Isoform Transcripts | ||||
| Academic/Govt. | Commercial | |||
|---|---|---|---|---|
| Per run of 8 | Per sample | Per run of 8 | Per sample | |
| Library Prep. + Sequencing (~12 Gb and ~500k reads/sample) | inquire | inquire | inquire | inquire |
| RNA Extraction3 | Not yet available | Not yet available | ||
| Standard PacBio Iso-Seq Pipeline | $1000/project | $1500/project | ||
Notes/Details
1. One control well is required per 96-well PCR library plate, hence a maximum of 4x95=380 samples can be done together on one MiSeq run (190 for a 2X run) or 2x95=190 samples on a Sequel2 run (95 for a 2X run). Any batches >380/190 will need to be spread across multiple MiSeq/Sequel2 runs.
2. These are generally maximum raw read outputs you will receive, but there is inherent variability in runs, meaning averages can be closer to 30k/60k (MiSeq 1X/2X) or 12k/24k (Sequel 2 1X/2X).
3. Extra costs may be incurred if samples, due to their nature, require more specialized (length, cost, etc.) extraction procedures.
4. & 5. Details of our amplicon and metagenomics pipelines are available at https://github.com/mlangill/microbiome_helper, but a summary of the major deliverables clients will receive (analyses will require a "mapping file" from the clients containing any relevant metadata for the study) are available on our Protocols page.
Custom Bioinformatics: Additional bioinformatic analyses can be requested at an hourly rate or through research collaboration. Please contact us for more details.
Customs primers/amplicons: Beyond the 16S/18S/ITS amplicons offered "in-stock", essentially any PCR amplicon (max. size ~550 bp, best ~450-500 bp for MiSeq; no size limit for Sequel 2) can be adapted to our systems. Please consults us for primer design/protocol details. Pricing is as follows (one-time extra oligo ordering charge): $1050/1400/2100 for oligos for 1-95/96-190/191-380 samples for MiSeq; $350/700 for oligos for 1-95/96-190 samples for Sequel 2.
Processing time: Time to completion may be variable depending the amount of other partial projects in the queue - this generally no longer applies to amplicons as we are at capacity, but occurs more when submitting small numbers of samples for metagenomics runs (<<192 for NextSeq2000) or de novo genomes (<<48 for Sequel2).
Payment: Payment is required within 30 days of receiving your invoice, only after completion of work (ie: delivery of data). All payments are final and no refunds are accepted.