Services/Pricing (updated Nov.2025 - Download PDF)


  • Dec.2024: NEW MiSeq i100 sequencer with XLEAP chemistry has arrived (1st in Canada), therefore the original MiSeqs have been retired.
  • Mar.2025: NEW PacBio Vega sequencer operational Apr.1st (1st in Canada), the original Sequel2 has been retired.
  • Nov.2025: Due to the new MiSeq i100 and PacBio Vega, along with cost-saving on the NS2K, all sequencing services have been updated to reflect higher quality+outputs of the new machines and new reduced pricing for almost all.

Note: All prices listed are in Canadian Dollars (CAD). GST/HST will be added to all Canadian orders. A fee of $25/shipment to cover Customs costs will be added to all international orders.

For a listing of currently available Amplicon Targets (and primer sets), see our Protocols page here.

 16S/18S/ITS Short Amplicons (per sample, per amplicon) - MiSeq i100 (2x300 bp PE)

Academic/Govt. Commercial
Per sample1 Per sample1
Library Preparation + Sequencing (max. 75k reads/sample2) $30 $20 $40 $30
      or 2X depth (max. 150k reads/sample2) $45 $30 $55 $40
DNA Extraction3 $35 $45
Standard Bioinformatics Analysis Pipeline4 $1000/project $1500/project

 16S/18S/ITS Full-Length Amplicons (per sample, per amplicon) - PacBio Vega (long reads)

Academic/Govt. Commercial
Per sample1 Per sample1
Library Preparation + Sequencing (max. 35k CCS=HiFi reads/sample2) $35 $30 $45 $40
      or 2X depth (max. 70k HiFi reads/sample2) $65 $55 $75 $65
DNA Extraction3 $35 $45
Standard Bioinformatics Analysis Pipeline4 $1000/project $1500/project

 Client-Prepared Pool of Amplicons (per whole run) - MiSeq i100 or Vega

Academic/Govt. Commercial
Per run Per run
MiSeq i100 5M 2x150 bp Run (~5 M PE reads & 1.5 Gb) - typically for ~75 samples $1700 $2000
MiSeq i100 5M 2x300 bp Run (~5 M PE reads & 3 Gb) - typically for ~75 samples $2300 $2500
MiSeq i100 25M 2x150 bp Run (~25 M PE reads & 7 Gb) - typically for ~380 samples $2800 $3200
MiSeq i100 25M 2x300 bp Run (~25 M PE reads & 15 Gb) - typically for ~380 samples $3300 $3600
Vega Whole Cell Run (~6 M HiFi reads, variable Gb) - typically for ~190 samples $4000 $5000
Note: For clients wishing to use part of a 25M run with a prepared pool of amplicons, simply divide 380 by your sample # then multiply by the above price to determine your pool cost (this is for a 1X depth, simply double the amount for 2X).

 Client-Prepared Pool of Metagenomes (per whole run) - NextSeq2000 (2x150 bp PE) or Vega

Academic/Govt. Commercial
Per run Per run
NextSeq2000 P1 cell Run (~100 M PE reads = 200 M single reads & 30 Gb) $4000 $5000
NextSeq2000 P2 cell Run (~400 M PE reads = 800 M single reads & 120 Gb) $7500 $9000
NextSeq2000 P3 cell Run (~1.2 B PE reads = 2.4 B single reads & 360 Gb) $11000 $12000
Vega Whole Cell Run (~6 M HiFi reads, variable Gb) $4000 $5000
Note: For clients wishing to use part of a P3 run with a prepared pool of metagenomes, simply divide 192 by your sample # then multiply by the above price to determine your pool cost (this is for a 1X depth).
 

 Viral/Phage Genomes (~1000X typical ~150 kb genome) - MiSeq i100 (2x300 bp PE) - De novo Assembly

Academic/Govt. Commercial
Per genome Per genome
Library Prep. + Sequencing (~0.25 M PE reads = 0.5 M single reads & 150 Mb/genome) $200 $110 $300 $165
DNA Extraction3 $35 $45
Custom Bioinformatics Analysis inquire inquire

 Prokaryotic Genomes (~100X typical ~5 Mb genome) - MiSeq i100 (2x300 bp PE) - Resequencing

Academic/Govt. Commercial
Per genome Per genome
Library Prep. + Sequencing (~0.9 M PE reads = 1.8 M single reads & 540 Mb/genome) $500 $300 $600 $400
DNA Extraction3 $35 $45
Custom Bioinformatics Analysis inquire inquire

 Prokaryotic Genomes (~100X typical ~5 Mb genome) - PacBio Vega (HiFi long reads) - De novo Assembly

Academic/Govt. Commercial
Per genome Per genome
Library Prep. + Sequencing (~500 Mb HiFi/genome)5 $275 $200 $365 $300
DNA Extraction3 $35 $45
Custom Bioinformatics Analysis inquire inquire

 Fungal Genomes (~100X typical 30-40 Mb genome) - NextSeq2000 (2x150 bp PE) - Resequencing or Hybrid with PacBio

Academic/Govt. Commercial
Per genome Per genome
Library Prep. + Sequencing (~12 M PE reads = 24 M single reads & 3.6 Gb/genome) $260 $180 $340 $275
DNA Extraction3 $35 $45
Custom Bioinformatics Analysis inquire inquire

 Fungal Genomes (~100X typical 40 Mb genome) - PacBio Vega (long reads) - De novo Assembly

Academic/Govt. Commercial
Per genome Per genome
Library Prep. + Sequencing (~4 Gb HiFi/genome)5 $1200 $1600
DNA Extraction3 $35 $45
Custom Bioinformatics Analysis inquire inquire

 (Micro)Eukaryote Genomes (20 Gb HiFi per cell)5 - PacBio Vega (HiFi long reads) - De novo Assembly

Academic/Govt. Commercial
Per genome Per genome
Library Prep. + Sequencing (~10 Gb Hifi)5 $3000 $4000
      or whole cell (~20 Gb HiFi)5 $5500 $6500
DNA Extraction3 $35 $45
Custom Bioinformatics Analysis inquire inquire

 

 Metagenomes - NextSeq2000 (2x150 bp PE P3 cell) - Profiling and Contig Assembly

Academic/Govt. Commercial
Per sample Per sample
Library Prep. + Sequencing (~6 M PE reads = 12 M single reads & 1.8 Gb/sample) $180 $120 $240 $180
      or 2X depth (~12 M PE reads = 24 M single reads & 3.6 Gb/sample) $260 $180 $340 $275
      or 4X depth (~24 M PE reads = 48 M single reads & 7.2 Gb/sample) $420 $325 $560 $500
      or 6X depth (~36 M PE reads = 72 M single reads & 11 Gb/sample) $590 $450 $790 $700
      or 8X depth (~48 M PE reads = 96 M single reads & 14 Gb/sample) $760 $600 $1000 $900
      or 12X depth (~72 M PE reads = 144 M single reads & 22 Gb/sample) $1080 $875 $1450 $1350
      or 16X depth (~96 M PE reads = 192 M single reads & 29 Gb/sample) $1400 $1150 $1900 $1750
      or 24X depth (~144 M PE reads = 288 M single reads & 43 Gb/sample) $2050 $1700 $2800 $2600
      or 32X depth (~192 M PE reads = 384 M single reads & 58 Gb/sample) $2700 $2300 $3600 $3500
DNA Extraction3 $35 $45
Standard Bioinformatics Analysis Pipeline4 $1000/project $1500/project

 Metagenomes - PacBio Vega (HiFi long reads) - Shallow Profiling

Academic/Govt. Commercial
Per sample Per sample
Library Prep. + Sequencing (~500 Mb HiFi/sample)5 $275 $200 $365 $300
DNA Extraction3 $35 $45
Custom Bioinformatics Analysis inquire inquire

 Metagenomes - PacBio Vega (HiFi long reads) - MAG Assembly

Academic/Govt. Commercial
Per sample Per sample
Library Prep. + Sequencing (~10 Gb HiFi/sample)5 $3000 $4000
      or whole cell (~20 Gb HiFi/sample)5 $5500 $6500
DNA Extraction3 $35 $45
Custom Bioinformatics Analysis inquire inquire

 

 (Meta)transcriptomes - NextSeq2000 (2x150 bp PE P3 cell) - Total Stranded RNA w/rRNA Depletion

Academic/Govt. Commercial
Per sample Per sample
Library Prep. + Sequencing (~24 M PE reads = 48 M single reads & 7.2 Gb/sample) $560 $425 $725 $650
RNA Extraction3 Not yet available Not yet available
Standard Bioinformatics Analysis Pipeline4 $1000/project $1500/project

 Iso-Seq - PacBio Vega (HiFi long reads) - Full-Length Isoform Transcripts

Academic/Govt. Commercial
Per sample Per sample
Library Prep. + Sequencing (~2.5 Gb HiFi and ~800k reads/sample)5 inquire inquire
RNA Extraction3 Not yet available Not yet available
Standard PacBio Iso-Seq Pipeline $1000/project $1500/project

 


Notes/Details

1. One control well is required per 96-well PCR library plate, hence a maximum of 4x95=380 samples can be done together on one MiSeq run (190 for a 2X run) or 2x95=190 samples on a Vega run (95 for a 2X run). Any batches >380/190 will need to be spread across multiple MiSeq/Vega runs.

2. These are generally maximum raw read outputs you will receive: there is inherent variability in runs and the performance of samples, depending on their quality, so expect a potential range of outputs as there is no way to guarantee the performance of complex DNA samples.

3. Extra costs may be incurred if samples, due to their nature, require more specialized (length, cost, etc.) extraction procedures.

4. Details of our amplicon and metagenomics pipelines are available at https://github.com/mlangill/microbiome_helper, but a summary of the major deliverables clients will receive are available on our Protocols page. Analyses will require a "mapping file" from the clients containing any relevant metadata for the study.

5. These Gb values are for the estimated HiFi (CCS) reads obtained and are dependent upon the final size of the library inserts, which can vary depending on the quality of the DNA and resulting library success.

Custom Bioinformatics: Additional bioinformatic analyses can be requested at an hourly rate or (more rarely due to limited resources) through research collaboration. Please contact us for more details.

Customs primers/amplicons: Beyond the 16S/18S/ITS amplicons offered "in-stock", essentially any PCR amplicon (max. size ~550 bp, best ~450-500 bp for MiSeq; no size limit for Vega) can be adapted to our systems. Please consults us for primer design/protocol details. Pricing is as follows (one-time extra oligo ordering charge): $1050/1400/2100 for oligos for 1-95/96-190/191-380 samples for MiSeq; $350/700 for oligos for 1-95/96-190 samples for Vega.

Processing time: Time to completion may be variable depending the amount of other partial projects in the queue - this generally does not apply to amplicons as we have enough capacity to complete runs weekly, but occurs more when submitting small numbers of samples for metagenomics runs (<<192 for NextSeq2000) or de novo genomes (<<48 for Vega).

Payment: Payment is required within 30 days of receiving your invoice, only after completion of work (ie: delivery of data). All payments are final and no refunds are accepted.