Services & Pricing

Prices include library preparation + sequencing = from DNA/RNA to results!

Announcements

Nov. 2025: Due to the new MiSeq i100 and PacBio Vega, along with cost-saving on the NS2K, all sequencing services have been updated to reflect higher quality+outputs of the new machines and new reduced pricing for almost all.
Mar. 2025: NEW PacBio Vega sequencer operational Apr.1st (1st in Canada), the original Sequel2 has been retired.
Dec. 2024: NEW MiSeq i100 sequencer with XLEAP chemistry has arrived (1st in Canada), therefore the original MiSeqs have been retired.

Amplicon Sequencing (16S/18S/ITS etc) - Price per sample, per amplicon

Short amplicons are sequenced on our Illumina MiSeq i100, using 2x300 bp paired-end reads. Full-length amplicons are sequenced on our PacBio Vega. For a listing of currently available Amplicon Targets (and primer sets), see our Protocols page. Custom primer/amplicon options are available (see Notes on page bottom).

Amplicon Type	Depth	Reads¹	Academic/Govt. Price (CAD)	Commercial Price (CAD)
MiSeq i100 - Short	1X	Max. 75k PE	$20	$30
MiSeq i100 - Short	2X	Max. 150k PE	$30	$40
Vega - Full Length	1X	Max. 35k HiFi	$30	$40
Vega - Full Length	2X	Max. 70k HiFi	$55	$65

Metagenome Sequencing - Price per sample

Metagenomic libraries are sequenced on our Illumina NextSeq2000 and PacBio Vega sequencers.

Metagenomes - NextSeq2000 (2x150 bp PE P3 cell) - Profiling and Contig Assembly
Depth	PE Reads (M)¹	Yield (Gbp)¹	Academic/Govt. Price (CAD)	Commercial Price (CAD)
1X	6	1.8	$120	$180
2X	12	3.6	$180	$275
4X	24	7.2	$325	$500
6X	36	11	$450	$700
8X	48	14	$600	$900
12X	72	22	$875	$1,350
16X	96	29	$1,150	$1,750
24X	144	43	$1,700	$2,600
32X	192	58	$2,300	$3,500

Metagenomes - PacBio Vega (HiFi long reads) - Shallow Profiling and MAG Assembly
Method	Depth	Yield¹	Academic/Govt. Price (CAD)	Commercial Price (CAD)
Shallow Profiling	1/48 Cell	0.5 Gbp HiFi	$200	$300
MAG Assembly	Half Cell	10 Gbp HiFi	$3,000	$4,000
MAG Assembly	Whole Cell	20 Gbp HiFi	$5,500	$6,500

Whole Genome Sequencing & Resequencing - Price per genome

The IMR offers high-quality WGS services for organisms ranging from small viral/phage genomes to complex micro-eukaryotes. Below are the service options grouped by target organism.

Viral/Phage Genomes (~150 kbp size, ~1000x coverage)
Method	Platform	Read Length	Reads¹	Yield¹	Academic/Govt. Price (CAD)	Commercial Price (CAD)
De novo Assembly	MiSeq i100	2x300 bp	0.25 M PE	150 Mbp	$110	$165

Prokaryotic Genomes (~5 Mbp size, ~100x coverage)
Method	Platform	Read Length	Reads¹	Yield¹	Academic/Govt. Price (CAD)	Commercial Price (CAD)
Resequencing	MiSeq i100	2x300 bp	0.9 M PE	540 Mbp	$300	$400
De novo Assembly	Vega 1/48 Cell	HiFi Long	125k	500 Mbp	$200	$300

Fungal Genomes (30-40 Mbp size, ~100x coverage)
Method	Platform	Read Length	Reads¹	Yield¹	Academic/Govt. Price (CAD)	Commercial Price (CAD)
Reseq or Hybrid	NextSeq2k(2X)	2x150 bp	12 M PE	3.6 Gbp	$180	$275
De novo Assembly	Vega 1/5 Cell	HiFi Long	1 M	4 Gbp	$1,200	$1,600

(Micro)Eukaryote Genomes - PacBio Vega (HiFi long reads)
Method	Platform	Read Length	Reads¹	Yield¹	Academic/Govt. Price (CAD)	Commercial Price (CAD)
Reseq. or Hybrid	NextSeq2k(12X)	2x300 bp	72 M PE	22 Gbp	$875	$1,350
De novo Assembly	Vega - Half Cell	HiFi Long	0.5 M	10 Gbp	$3,000	$4,000
De novo Assembly	Vega - Full Cell	HiFi Long	1 M	20 Gbp	$5,500	$6,500

Client-Prepared Pool Sequencing - Price per run

This service is for clients who have fully prepared their own libraries (Amplicon or Metagenomic) with barcodes+adapters and pooled them, ready for direct sequencing on our Illumina platforms. Pricing is per whole sequencer run, but partial runs can be purchased on a pro-rated basis².

Client-Prepared Pools - MiSeq i100 (amplicons) and NextSeq2000 (metagenomes) - per whole run
Pool Type	Platform	Read Length	Reads¹	Yield (Gbp)¹	Samples/Run	Academic/Govt. Price (CAD)	Commercial Price (CAD)
Amplicons	MiSeq 5M	2x150 bp	5 M PE	1.5	~75	$1,700	$2,000
Amplicons	MiSeq 5M	2x300 bp	5 M PE	3	~75	$2,300	$2,500
Amplicons	MiSeq 25M	2x150 bp	25 M PE	7	~380	$2,800	$3,200
Amplicons	MiSeq 25M	2x300 bp	25 M PE	15	~380	$3,300	$3,600
Amplicons	Vega Cell	HiFi Long	6 M	variable	~190	$4,000	$5,000
Metagenomes	NS2K P1	2x150 bp	100 M PE	30	~24	$4,000	$5,000
Metagenomes	NS2K P2	2x150 bp	400 M PE	120	~64	$7,500	$9,000
Metagenomes	NS2K P3	2x150 bp	1.2 B PE	360	~192	$11,000	$12,000
Metagenomes	Vega Cell	HiFi Long	6 M	variable	~48	$4,000	$5,000

(Meta)Transcriptome & Isoform Sequencing - Price per sample

During Metatransciptomic and RNAseq library prep, samples undergo rRNA depletion and finish as Total Stranded RNA. They are sequenced on our NextSeq2000 (2x150 bp PE). Isoform sequencing (Iso-Seq) is performed on our PacBio Vega for full-length transcripts.

Method	Platform	Reads¹	Yield¹	Academic/Govt. Price (CAD)	Commercial Price (CAD)
(Meta)Transcriptomes	NextSeq	24 M PE	7.2 Gbp	$425	$650
Iso-Seq	Vega	800k	2.5 Gbp HiFi	inquire	inquire

DNA Extraction (optional/extra fee) - Price per sample

The IMR offers nucleic acid extraction services for most standard samples (ex: soil, stool, cultures) and select low-biomass samples - visit our Microbiome Sequencing Wizard for guidance on which samples types we accept. Prices for these services are separate from sequencing and extra costs may be incurred if samples, due to their nature, require more specialized (length, cost, etc.) extraction procedures.

Service	Academic/Govt. Price (CAD)	Commercial Price (CAD)
DNA Extraction	$35	$45

Bioinformatics Services (optional/extra fee) - Price per project/analysis

Complete details of our amplicon and metagenomics pipelines are available on our Microbiome Helper website, but a summary of the major deliverables clients will receive are available on our Protocols page. Analyses will require a "mapping file" from clients containing any relevant metadata for the study and are charged once per project submission (ie: per time we have to run the analysis).

Service	Academic/Govt. Price (CAD)	Commercial Price (CAD)
Standard Bioinformatics Analysis Pipeline	$1000/project	$1500/project
Custom Bioinformatics	inquire	inquire

Notes

1. These are generally maximum raw read/base outputs you will receive: there is inherent variability in runs and the performance of samples, depending on their quality, so expect a potential range of outputs as there is no way to guarantee the performance of complex DNA samples. Additionally for PacBio, the Gb values are for the estimated HiFi (CCS) reads obtained and are dependent upon the final size of the library inserts, which can vary depending on the quality of the DNA and resulting library success.

2. For clients wishing to use part of a 25M run with a prepared pool of amplicons, simply divide 380 by your sample # then multiply by the above price to determine your pool cost (this is for a 1X depth, simply double the amount for 2X). Similarly, for clients wishing to use part of a P3 run with a prepared pool of metagenomes, simply divide 192 by your sample # then multiply by the above price to determine your pool cost (this is for a 1X depth).

Final Pricing: GST/HST will be added to all Canadian orders. A fee of $25/shipment to cover Customs costs will be added to all international orders.

Max Samples per Run: For amplicons, one control well is required per 96-well PCR library plate, hence a maximum of 4x95=380 samples can be done together on one MiSeq run (190 for a 2X run) or 2x95=190 samples on a Vega run (95 for a 2X run). Any batches >380/190 will need to be spread across multiple MiSeq/Vega runs.

Customs primers/amplicons: Beyond the 16S/18S/ITS amplicons offered "in-stock", essentially any PCR amplicon (max. size ~550 bp, best ~450-500 bp for MiSeq; no size limit for Vega) can be adapted to our systems. Please consults us for primer design/protocol details. Pricing is as follows (one-time extra oligo ordering charge): $1050/1400/2100 for oligos for 1-95/96-190/191-380 samples for MiSeq; $350/700 for oligos for 1-95/96-190 samples for Vega.

Custom Bioinformatics: Additional bioinformatic analyses can be requested at an hourly rate or (more rarely due to limited resources) through research collaboration. Please contact us for more details.

Processing time: Time to completion may be variable depending on the amount of other partial projects in the queue - this generally does not apply to amplicons as we have enough capacity to complete runs weekly, but occurs more when submitting small numbers of samples for metagenomics runs (<192 for NextSeq2000) or de novo genomes (<48 for Vega).

Payment: Payment is required within 30 days of receiving your invoice, only after completion of work (ie: delivery of data). All payments are final and no refunds are accepted.